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Process for the continuous production of cellulases by a filamentous fungus using a carbon substrate obtained from an acid pretreatment
Process for the continuous production of cellulases by a filamentous fungus using a carbon substrate obtained from an acid pretreatment
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机译:用丝状真菌用酸预处理得到的碳底物连续生产纤维素酶的方法
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摘要
A process for the production of cellulases and hemicellulases using a strain of a filamentous fungus in a stirred and aerated bioreactor, in at least two phases, a phase a) for growth of said strain in the presence of at least one carbonaceous growth substrate in a closed reactor, a phase b) for the continuous production of cellulases, in which at least one carbonaceous inducer substrate is supplied at a supply rate which is constant over a period of at least more than 200 h, said carbonaceous substrate being at least one aqueous hemicellulosic hydrolysate solution obtained from an acid pre-treatment of a lignocellulosic substrate, said aqueous hemicellulosic hydrolysate solution not undergoing prior sterilization and not undergoing pH rectification, being operated at a dilution rate in the range 0.001 to 0.02 h−1.
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机译:一种在至少两个阶段中在搅拌和充气的生物反应器中使用丝状真菌菌株生产纤维素酶和半纤维素酶的方法,阶段a)用于在至少一种碳质生长底物存在下在至少一个阶段中生长所述菌株。密闭反应器,阶段b),用于连续生产纤维素酶,其中至少一种碳质诱导物底物以至少在超过200小时的时间内恒定的供给速率供给,所述碳质底物是至少一种水性通过对木质纤维素底物进行酸预处理获得的半纤维素水解物溶液,所述半纤维素水解物水溶液未经过事先灭菌且未经过pH精馏,其稀释速率为0.001至0.02 h -1 晚餐>。
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