METHOD FOR THE CONTINUOUS PRODUCTION OF CELLULASES BY A FILAMENTOUS FUNGUS USING A CARBON SUBSTRATE OBTAINED FROM AN ACID PRETREATMENT

摘要

The present invention relates to a process for the production of cellulases and hemicellulases by a strain belonging to a filamentous fungus, in a stirred and aerated bioreactor comprising at least two phases, a phase a) of growth of said strain in the presence of at least one carbonaceous growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbonaceous growth substrate of between 10 and 90 g / L, a phase b) continuous production of cellulases in which at least one inducing carbonaceous substrate is supplied at a feed rate at least constant for a duration at least greater than 200 h, said carbon substrate being at least one aqueous solution of hemicellulosic hydrolyzate resulting from an acid pretreatment of a lignocellulosic substrate, said aqueous solution of hemicellulosic hydrolyzate not undergoing prior sterilization and no pH correction, said pH of the solution aq ueuse being between 0.5 and 3, the mass of the reaction volume being kept constant by drawing off a fraction of said reaction volume, said phase b) operating at a dilution rate of between 0.001 and 0.02 h-1.