(self orientation method and composition of mother Mason chiama)

摘要

Transmission refers to the method and composition of self-contained plateau stem cells (MSCs). The components include the compounds described in this application, which can guide MCC to express cell surface self-directed adhesion molecules, such as CD11a, and help MCC to adhere more firmly in the test tube section flow test, 1. To increase the binding with e-selectina or ICAM-1, and / or to show anti-inflammatory activity through in vivo system management of cell therapy using human MSCs. Besides,In order to find out the small molecular compounds and enhance the self conducting function of MSC, a systematic detection method was introduced. 1. Claim 1: methods for treating patients with injuries and injuries, including (a) the stages of providing high altitude stem cells (MSc);(b) Sanitary and phytosanitary measures are in contact with an effective number of compounds, including compounds (1) in the formula,so as to generate a composition comprising pretreated MSC, where the compound is capable of enhancing a self-steering function in pretreated MSC relative to that of untreated MSC, where said contact optionally includes incubation of the MSC with the compound composition; and (b) optionally, washing the pretreated MSCs; (c) administering an effective amount of the composition comprising pretreated MSCs, to the subject; where the compound has the structure of the formula (1) where: R¹ is selected from the series consisting of hydrogen, C₁₋₄-alkyl, phenyl-C₁₋₄-alkyl- and heteroaryl-C₁₋₄-alkyl-,where phenyl and heteroaryl are unsubstituted or substituted with substituents of the series consisting of halogen, C₁₋₄-alkyl and C₁₋₄-alkyl-O-;R2 and R3 are independently selected,e. In the halogen series, c83218331;R⁴ is selected from the series consisting of hydrogen and C₁₋₄-alkyl; R¹⁰ is selected from the series consisting of hydrogen, C₁₋₆-alkyl and Het¹,where C₁₋₆-alkyl is unsubstituted or substituted with R²⁰,Where hete1 has not been replaced or replaced by R2,R¹¹ is selected from the series consisting of hydrogen and C₁₋₄-alkyl, or R¹⁰ and R¹¹ together are a divalent group selected from the series consisting of the groups - (CH₂) ₘ-C (R²²) (R²³) - ( CH₂) ₙ- and - (CH₂) ₙ-N (R²⁴) - (CH₂) ₙ-,where the (CH₂) ₘ and (CH₂) restos residues are unsubstituted or substituted with C₁₋₄-alkyl; R²⁰ is selected from the series consisting of R³⁰-O-,R³¹-N (R³²) -,H N-C (3DNH) -S-Billy Dini and Hutt3. Het 'was not replaced by three cubic meters;R: 1 from c832131;where phenyl and pyridinyl are unsubstituted or substituted with substituents of the series consisting of halogen, C₁₋₄-alkyl and C₁₋₄-alkyl-O-;R²² is selected from the series consisting of hydrogen and C₁₋₄-alkyl; R²³ is selected from the series consisting of hydrogen, C₁₋₄-alkyl, R³¹-N (R³²) - and R³¹-N (R³²) -C₁₋₄-alkyl-;R se selecciona de la serie que consiste en hidr geno C -alquilo piridinilo-C -alquilo- y R -N (R) -C -alquilo-R³⁰,R³¹ and R³² are selected, independently of each other,from the series consisting of hydrogen and C₁₋₄-alkyl; R³³ is selected from the series consisting of C₁₋₄-alkyl; Het¹ is a saturated monocyclic 4- to 7-membered heterocycle, comprising a ring nitrogen atom, which is bonded via a ring carbon atom; Het² is a saturated monocyclic, 4- to 7-membered heterocycle comprising one or two ring nitrogen atoms, which is bonded via a ring carbon atom or a ring nitrogen atom; heteroaryl is a 5-membered or 6-membered heterocyclic, monocyclic, aromatic,It consists of one or two identical or different ring atoms selected from the N, O and S series; a and B are selected independently from each other,In the series of 0, 1 and 2, m and N are selected independently,A series of 1 and 2. Claim 8: the method of claim 1, wherein the composite method of item (1),The chemical name of the compound is 3 - (8-carbamate-6, 7, 8, 9-tetrahydro-pyridine [1, 2-A] india-10-il) - 4 - (1-methyl-1h-india-3-il) - Pyridine-2, 5-dioxy; the formula structure of the compound is (2). Claim 19: a compound composed of an in vitro purified MSc and an effective number of compounds, as described in the present application, which can enhance the self directing function of the MSC,The compound can be selected from formula (1) or any formula (2) to (20). Requirement 20: a system determination method for identifying a small molecular compound, which can improve a selective MSc function, including all stages: (a) providing candidate components including small molecular candidate components; (b) providing MSc; (c) How to deal with sanitary and phytosanitary measures and make them have candidate components to produce treated sanitary and phytosanitary measures; (d) how to measure the characteristics of treated sanitary and phytosanitary measures;This characteristic includes the expression of one or more cell surface molecules in vitro, which can promote the self-directed function, the in vitro adhesion with no visible effect concentration treated in the slice flow test, and the anti-inflammatory activity managed by the in vivo system in the animal inflammatory model; (e) Compare one or more sanitary and phytosanitary characteristics to a negative control sanitary and phytosanitary characteristic, in which the negative control sanitary and phytosanitary measures are not treated or treated, while the negative control candidate components cannot enhance the self-management function of sanitary and phytosanitary measures;Y (f) recognizes small molecular compounds, which can enhance the self directing function of MSC. In this function, the compounds of small molecules treated in the negative control of MSC show higher performance of one or more cell surface molecules that can provide one self directing function, In the section flow test, the in vitro adhesion was increased, the activity of autoimmune disease was reduced in the animal model of in vivo system management, and the anti-inflammatory activity of in vivo system management was increased in the animal model of inflammation. Requirement 24: a purified MSc in vitro culture,Among them, Morgan Stanley Capital International said that the cell area of CD11a is higher than that of the planted Morgan Stanley Capital International, whose largest cell area is endogenous and not designed. 13. Claim 27: a method for the development of therapeutic components of pre-treatment sanitary and phytosanitary products capable of enhancing self-management functions, including: (a) the provision of sanitary and phytosanitary products at all stages of the population; (b) The MSc is explained by contacting the MSc composed of the compound in the application, in which the compound can enhance the self directing function of the MSc; in the compound,The alternative is formula (1) or any formula (2) to (20),The self directing function is: (1) to enhance the expression ability of cell surface molecules, so that they can play the self directing function, in which the cell surface molecules are CD11a optionally; (2) to enhance the viscosity of MSC to the test tube in the slice flow test, (III) enhance the combination of e-selectina or ICAM-1, and (IV) increase the self-directed and / or anti-inflammatory activities of sanitary and phytosanitary measures in combination with the in vivo system management of sanitary and phytosanitary measures in animal models of inflammation, (c) prepare single dose or multi dose arias, (d) Optionally,3. Freeze the pre-treatment sanitary and phytosanitary measures to produce the pre-treatment sanitary and phytosanitary measures and thus the therapeutic components of the pre-treatment sanitary and phytosanitary measures.