process of intra-lesional treatment of leishmania brasiliensis infected hamsters with astronium fraxinifolium

摘要

process of intra-lesional treatment of leishmania brasiliensis infected hamsters with astronium fraxinifolium. animal infection protocol: to evaluate the leishmanicidal capacity of the ethanolic extract of leaves of a. fraxinifolium three groups with 10 animals in each were used. the groups were divided as follows: group 1 (e) infected and treated with the ethanolic extract of the leaf (2opl intra-lesion via the right hind paw of each animal groups 2 (g) infected and treated with glucantime (2opl via right hind paw of each animal group 3 (c) infected but not treated, inoculation was performed in the ventral region of the left hind paw of each animal, paw thicknesses were measured weekly with the aid of a digital micrometer and the animals were weighed weekly also animal treatment protocol: on the 20th day after infection, the first group underwent a treatment with 15 daily applications of a.fraxinifolium (20 pl volume) ethanolic leaf extract subcutaneously The second group was treated with 40 pl of meglumine antimoniate (20 mglkg), also subcutaneously, the third group was not treated, the hind paw thickness was measured weekly. and the heavy animals, also weekly. On the 5th day after the end of treatment, the animals were euthanized for parasite load evaluation (pp). The treatment with plant samples under the proposed conditions showed efficacy similar to standard drug and with less side effect, evaluated by body mass and physical aspects.