Methods and compositions for the recombination of the gene of Agrobacterium tumefaciens strains deficient

摘要

Methods and compositions for recombination deficient strains of Agrobacterium tumefaciens gene. The present invention relates to new compositions and methods for the production and use of strains of Agrobacterium tumefaciens (for example, LBA4404) that are deficient activity of RecA strain on the dam.The combinations with other genes in strains of Agrobacterium tumefaciens, disabled people are also described. Specifically, are provided two RecA copies less strains, uia777 where the gene of resistance to chloramphenicol interrupts the RecA gene and uia770 where the gene for kanamycin resistance gene disrupts the RecA.