Construction of DNA, the expression system, a host cell, as well as a method to express a gene involved in cell walls during secondary wall deposition in the secondary

摘要

"DNA molecules coding quitinase from cotton expressed preferentially in cells of secondary walls during the deposition of secondary wall and a corresponding promoter.The present invention relates to a nucleic acid molecule encoding a kinase isolated cotton and its endogenous promoter, which are preferentially expressed in cells of secondary wall deposition on the walls during secondary.The polypeptide encoded by are also revealed the nucleic acid molecule, a DNA construct obtained by chaining the isolated nucleic acid molecule with a promoter, the DNA construct incorporated in an expression system, a host cell, a plant, or a seed of a plant.The present invention also refers to a DNA construct obtained by chaining the promoter isolated with a second DNA as well as expression systems, host cells, plants or plant seeds containing the DNA construct.Methods are also disclosed for conferring resistance to insects and fungi, regulate the content of cellulose fiber, and methods for expressing a gene preferentially in cells during secondary wall deposition of secondary wall.