A PROCESS FOR THE ISOLATION AND CHARACTERIZATION OF AN ALPHA -GLUCOSIDE INHIBITOR FROM LEAVES OF TINOSPORA CORDIFOLIA MIERS, USEFUL FOR NON-INSULIN DEPENDENT DIABETIC TREATMENT

摘要

The present invention provides a process for isolation of an alpha glucosidase inhibitor comprising saponarin, preferably obtained from the leaves of Tinospora cordifolia, Miers, useful for non-insulin dependent diabetic treatment. The inhibitor obtained by the said process, competitively inhibits the hydrolyzing activities of maltase and sucrase of intestinal and microbial origin. The inhibition constant (Ki) for the said alpha glucosidase inhibitor comprising saponarin is in the range of 5.0 - 7.4�g/ml at pH 6.5 at temperature ranging between 37 -50�C. Saponarin isolated from the process can be used as lead molecule for the development of chemically modified saponarin or structural analogue of saponarin for preparation of effective alpha glucosidase inhibitor useful for the treatment of non insulin dependent diabetes.