METHODS FOR ESTIMATING ANTIBIOTIC SUSCEPTIBILITY AND ANTIBIOTIC SENSITIVITY PROFILE OF MICROORGANISMS AND DEVICES THEREFOR

摘要

[0063] In one aspect the invention provides a method for estimating antibiotic susceptibility of microorganisms wherein the method comprises estimating an initial concentration of microorganisms in the sample followed by adjusting the concentration of the microorganisms such that it is in a predetermined range to provide an adjusted sample. The predetermined range may be from about 1 x 104 cells/mL to about 1 x 107cells/mL. Subsequently the adjusted sample is exposed to an antibiotic under predetermined conditions to provide an exposed sample following which the concentration of microorganisms is estimated which is used to estimate the antibiotic susceptibility of the microorganism. The method of the invention is capable of being extended to estimating antibiotic sensitivity profile by exposing the adjusted to different antibiotics at the same concentration or each at various concentrations. Kits and devices based on the methods of the invention are also described herein