METHOD COMPOSITION AND KIT OF VISUALIZATION AND CHARACTERIZATION OF THE NERVOUS SYSTEM BY COMBINING THE STAINING FOR METAL IMPREGNATION AND IMMUNOHISTOCHEMISTRY

摘要

A method of visualization and characterization of the nervous system which combines two techniques for tissue staining mercuric impregnation and immunohistochemistry is described. The method disclosed makes compatible one with the other procedures of staining by carrying them out on tissues subjected to adequate fixing procedures. Thus the method makes feasible to combine morphometric analysis which can typically be carried out with mercuric impregnation with antigenic and/or neurochemical cellular characterization which can typically be obtained with immunohistochemistry. The method has proven simple reliable and suitable for both conventional microscopy and confocal laser scanning microscopy.