“A recombinant chimeric protein r-CPAE for effective protection against enterotoxigenic Clostridium perfringens�

摘要

A recombinant bivalent chimeric gene r-CPAE encoding fusion protein for effective protection against Clostridium perfringens The present invention provides for a recombinant bivalent chimeric gene r-cpae (738 bp) inlcuding C domain (1030 to 1374 bp) region of C. perfringens alpha toxin (a total of 345 bp), Glycine linker sequence (a total of 45 bp), and C domain (732 to 1079 bp) region of C. perfringens enterotoxin (a total of 348 bp). The chimeric gene encodes a 26.6kDa bivalent fusion protein encompassing specific portions of alpha toxin and enterotoxin of Clostridium perfringens. The recombinant bivalent fusion protein encompassing the C- terminal binding domains of alpha and enterotoxin is used as an immunogen to elicit protective immune response and also as an effective candidate vaccine molecule against lethal Clostridium perfringens infections. Figure 1