HUMANIZED ANTI HUMAN EPIDEMIC GROWTH FACTOR RECEPTOR ANTIBODY AND ENCODING GENE AND APPLICATION THEREOF

摘要

Provided are a humanized anti-human epidemic growth factor receptor (EGFR) antibody and an encoding gene and an application thereof. According to the present invention, a single-chain antibody resisting the human EGFR is screened from a complete synthesis type single-chain human antibody library, and based on an obtained variable region gene sequence of the antibody, complete human monoclonal antibodies are constructed. The affinity of the antibody for the human EGFT is not less than InM, and the affinity of the mutant of the antibody for the human EGFR is not less than 10 nM. The antibody has good biological activity for inhibiting the tumor growth of the EGFR high-expressing cell A431 tumor-bearing model mouse.