KALANCHOE FLAMMEA EXTRACT WITH ETHYL ACETATE, THE FRACTIONS AND MAJOR COMPOUNDS THEREOF FOR THE TREATMENT OF PROSTATE CANCER.

摘要

The present invention refers to a kalanchoe flammea extract with ethyl acetate (KfAcOEt-COP), the fractions and major compounds thereof, which may be used as cytotoxic or apoptosis inducers against prostate cancer, considering that Docetaxol is a more efficient drug, the KfAcOEt-COP extract (which has fractions and major compounds) shown to have more advantages than Docetaxol. First, a phyto-chemical study was performed where alkaloids, sterols and cardiac glycosides were found. Referring to cytotoxicity, the KfAcOEt-COP effect was evaluated as well as its fractions over the viability of PC3, LNCaP, PrEC and lymphocytes; these may exert a doses response effect after 24 h of exposition. Genotoxic studies in vitro and in vivo of KfAcOEt-COP and its fractions were performed, where no toxicity was found. The KfAcOEt-COP extract has evidence of inducing apoptosis after 6 h, the PC3 exposed to the extract showing an overproduction of ROS. The activation of caspases is intrinsically measure d, inducing the fragmentation of the DNA, showing a high level of excision of PARP-1 and inducing cell arrest in the synthesis phase. In an animal model, KfAcOEt-COP is able to inhibit the tumor growth in CaP cells.