New bis(2,2'-bitienylo)-(4-hydroxyphenyl)methane biotin ester, process for its preparation, and the ester containing polymer layer recognition and its application to the detection and/or determination of oligonucleotides

摘要

the subject invention is bis (2,2 '- bitienylo) - (4 - hydroxyphenyl) metanowy biotin ester of formula 1 and its manufacture.for this purpose, the solution of 2,2 '- bisbitiofenu in protycznym organic solvent is subjected to reaction with 4 - hydroksybenzaldehydem and with perchloric acid to produce bis (2,2' - bitienylo) - (4-hydroxyphenyl) methane, which then undergo reaction with d - biotynu0105 in the presence of l - ethyl - 3 - (3 - dimetoiloami well - propyl) karbomiimidu in aerotycznym organic solvent and is obtained in model 1.the invention also concerns polymer layer pattern which is a polymer containing biotin and how to receive this layer. the way is that the relationship of model 1 is elektropolimeryzacji with solution of acetonitrile and ethanol, which leads to the establishment of the polymer surface electrodes.in turn, this modifies the first layer in a solution of the polymer with the stationary protein, protein, after which the solution of the oligonucleotide biotynowanego pattern to produce polymer layer pattern. in addition, crystalline quartz resonator, in which the bus electrode is deposited polymer layer in a manner jw.shall be mounted in a magnetic frame flow-through microbalance kwarcowej where modifies the layer by passing the solution of protein, and then the solution of the oligonucleotide biotynowanego pattern to produce polymer layer pattern.the invention also includes the use of this layer as a chemical sensor element pattern piezograwimetrycznego and impedimetrycznego for selective detection and / or marking of the oligonucleotide of the oligonucleotide complementary pattern.