Eukaryotic expression vector encoding the human basic fibroblast growth factor, method for obtaining it and application

摘要

the subject of the invention is eukariotyczny expressive vector coding for human basic fibroblast growth factor isoform 18kda pcdna3 - 18fgf of general formula 1, based on a fragment cdna for bfgf 18kda.vector pcdna3 - 18fgf creates a plasmid containing cdna fragment pcdna3, with a total length of 506 pz, covering sequence from 461 to 948 pz according to sequence deposited in bank genes under number nm _ 002006.4 (bfgf), which is a limited additional sequences for xbai restrictive enzymes and the room.in addition, a cdna for bfgf 18kda provides sequence koduju0105cu0105 this gene at codon containing atg, a signal to start a process of translation and at codon tga is the signal termination of protein synthesis. the invention also relates method of production eukariotycznego vector pcdna3 - 18fgf of general formula 1.disclosed also apply eukariotycznego vector pcdna3 - 18fgf, for the manufacture of drug therapy for patients with critical limb ischemia intramuscular leg or with hard - goju0105cymi's wounds.