Method for obtaining exopolygalacturonase at the time of growing the culture of Penicillium chrysogenum mould

摘要

the subject of the invention is a method for egzo - polygalacturonase during husbandry of penicillium chrysogenum, whereby, in the first stage, preparing culture medium, in order to material containing pectic substances undergo sterilization at 121 oc for 20 minutes.then the sounds to a sterile saline solution (nh4) so4 and mgso4 - 7h2o concentration of 10 g / l, in the proportion of solution mixture of mass substrate 1 ml to 3 g substrate.in the second stage strain penicillium chrysogenum activated previously by transfer to a substrate agarowe and incubation at 21 - 24u00b0c by 10 - 14 days in sterile culture medium in a quantity of 1,0 ml 15 gram substrate breeding and production is periodic in 21 - 24u00b0c temperature for at least 5 dayswith between 45 and 55 hour breeding the following concentrated inorganic buffer: buffer fosforanowo - citrate or phosphate at ambient temperature of 0.5 - 1.0 ml for 3 g substrate, so that the culture ph ranging from 3.4 to 5.6, followed after 3 consecutive days isolated product in after staci proteins showing activity egzo - polygalacturonase.