METHOD FOR EARLY DIAGNOSIS OF PERSISTENT INTRACELLULAR INFECTIONS IN NEWBORNS

摘要

Method for early diagnosis of persistent intracellular infections in newborns, which is to identify deoxyribonucleic acid (DNA) of infectious agents using polymerase chain reaction (PCR), and to detect infection and identify persistent intracellular pathogens (PVZ) Herpes Simplex Virus 1, 2, Cytomegalovirus , Epstein-Barr Virus, Chlamydia Trahomatis, Toxoplasma gondii, using PCR Real-time, to identify the DNA of pathogens using biological INTRAOPERATIONAL material (saliva content of the esophagus, stomach, small and large intestine) children with congenital malformations (birth defects) gastrointestinal (GI) tract, this material is going to study a sterile disposable containers with lids that twisted, tsentryfuhuyetsya to get lit biomaterial extract, introduced in test tubes with lysis solution, thoroughly mixed on Vortex. Tsentryfuhuyetsya to the deposition of droplets is heated 5 minutes at 65 ° C and tsentryfuhuyetsya at 13 thousand. Rev. / Min., Isolated DNA is added to the tubes of reactive mixture contains species-specific primers and TaqF polymerase and performed amplification of detection in real time with the definition of quantitative and qualitative indicators using computer program.