Evaluation of Various Methods in the Diagnosis of G. Lamblia Infections. (Reannouncement with New Availability Information).

摘要

The merthiolate-Iodine- Formaldehyde-Ether Concentration (MIFC) technique, the Enterotest, Enzyme-linked-immunosorbent-assay (ELISA) forthe detection of specific anti-Giardia IgG in sera, ELISA and counterimmunoelectrophoresis (CIEP) for the detection of G. lamblia coproantigen were evaluated on 50 outpatient children, 3-12 years of age, suffering from diarrhea. Twenty-four out of 47 patients proved to be microscopically positive for G. lamblia infection by both MIFC technique and/or Enterotest. Of those positive cases, 54% were positive by MIFC technique, 92% by Enterotest, 25% by ELISA for the detection of anti-Giardia IgG in sera, 50% and 66.7% by ELISA and CIEP respectively for the detection of copro-antigen. Of those who were negative microscopically, 13% were positive by ELISA for the detection of anti-Giardia IgG in serum, while 87% were positive by both ELISA and CIEP for copro-antigen.