Late Embryonic Development and maturation in cold temperature.

摘要

We describe a method for Developing somatic embryos cotiledonarios coniferousIt includes cultivating Conifer somatic embryos precotiledonarios in a Development Environment at a temperature of between approximately 20 and approximately 22 during a First incubation period enough for at least a portion of precotiledona somatic Embryos Rivers developed somatic embryos cotiledonarios,And cultivate the cotiledonarios somatic embryos in the Development Environment at a temperature of between approximately 10c, 15C during approximately a Second incubation period for at least a portion of somatic embryos cotiledonarios Anatomical maturity.