Late Embryonic Development and maturation of Conifers to cold temperature.

摘要

We describe a method for the development of somatic embryos cotiledonares of conifers.The Method includes: Cultivating Conifer somatic embryos precotiledonares in - or - a Development Environment at a temperature of between approximately 20 and approximately 22 degrees Celsius during the first incubation period to be long enough so that at least a part of The precotiledonares somatic embryos and Somatic embryos develop and cultivate cotiledonares;The somatic embryos in cotiledonares - or - The Development Environment at a temperature between about 10 and about 15C during the second incubation period to be long enough so that at least a part of the cotiledonares to somatic Embryos Lcancen Anatomical maturity.