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CHROMATOGRAPHIC METHOD FOR ISOLATING AND PURIFYING HIGH-PURITY RECOMBINATION HUMAN SERUM ALBUMIN
CHROMATOGRAPHIC METHOD FOR ISOLATING AND PURIFYING HIGH-PURITY RECOMBINATION HUMAN SERUM ALBUMIN
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机译:层析纯化高纯度重组人血清白蛋白的方法
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摘要
Disclosed is a chromatographic method for isolating and purifying high-purity recombined human serum albumin, comprising: conducting cation-exchange chromatography on recombined human serum albumin, and adding alcohol to a buffer solution to remove endotoxin, so as to obtain a primary product I; under binding conditions, conducting anion/hydrophobic composite resin exchange chromatography on the primary product I to obtain an intermediate product II; conducting hydrophobic chromatography on the intermediate product II to obtain the target product, the purified high-purity recombined human serum albumin. The purity of the recombined human serum albumin obtained by isolating and purifying with the chromatographic method of the present invention is greater than 99.9999%, and the content of the endotoxin meets the criteria stipulated by Chinese pharmacopoeia.
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