regulatory T cells and their use in immunotherapy and suppression of autoimmune responses

摘要

A method for producing therapeutic CD4 + CD25 + CD62 + CD27 + regulatory T cells (Treg cells), wherein said method comprises: isolating a population of human CD4 + CD25 + suppressor T cells from a sample of CD4 + T cells using column purification that uses CD25 microspheres, where double column purification comprises purification in a first and a second column; and expanding in culture the CD4 + CD25 + Treg cells ex vivo with microspheres coated with antibodies to CD3 and CD28, and expanding in culture said isolated population of human CD4 + CD25 + Treg cells in the presence of IL-2, irradiated CD4 + feeder cell or the medium conditioning of irradiated CD4 + feeder cells, thus producing expanded cells in culture, wherein said expanded Treg cells in culture are CD62L + / CD27 + and are capable of inhibiting proliferation of CD4 + CD25-responsive T cells in a Mixed Lymphocyte Reaction assay ( MLR) for more than 90%.