CARTILAGE GEL FOR CARTILAGE REPAIR, COMPRISING CHITOSAN AND CHONDROCYTES

摘要

The present invention relates to a method for obtaining an implantable cartilage gel for tissue repair of hyaline cartilage comprising chitosan hydrogel particles and cells capable of forming hyaline cartilage, said method comprising a step of amplification of primary cells, in a three-dimensional structure comprising particles of physical chitosan hydrogel or chitosan derivative, then a step of re-differentiation and induction of extracellular matrix synthesis by said amplified cells, within the same structure three-dimensional, wherein said cells are primary articular chondrocytes and / or primary mesenchymal stem cells differentiated into chondrocytes. The present invention also relates to the cartilage gel thus obtained and its various uses for cartilage repair following traumatic shock or osteo-articular disease such as osteoarthritis. The invention also relates to a three-dimensional matrix comprising physical hydrogel particles of chitosan or of chitosan derivative, optionally supplemented with an anionic molecule such as hyaluronic acid or a hyaluronic acid derivative or a hyaluronic acid complex.