A secondary stage sample separation device (90) for separating at least a portion of a fluidic sample, wherein the secondary stage sample separation device (90) comprises a fluidic interface (89) configured for forming a detachable fluidic coupling between a primary stage sample separation device (10) and the secondary separation device so that the fluidic sample separated by the primary stage sample separation device (10) is fluidically supplyable to the secondary stage sample separation device (90) via the fluidic interface (89) for further separation, wherein the secondary stage sample separation device (90) is further configured for separating at least a portion of the supplied fluidic sample independent of a flow rate of the fluidic sample supplied from the primary stage sample separation device (10) at the fluidic interface (89).
展开▼