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Determination of the unbound free fatty acid profiles in biological and industrial specimens
Determination of the unbound free fatty acid profiles in biological and industrial specimens
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机译:测定生物和工业样品中未结合的游离脂肪酸谱
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摘要
A method for high throughput discovery of proteins fluorescently labeled at a cysteine residue and that undergo a change in fluorescence ratio at 2 wavelengths upon binding an unbound analyte is described. Probes are disclosed which are labeled at a cysteine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for characterization and measurement of hydrophobic species in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. A profile of unbound free fatty acids can be determined for an individual which can be used to determine the individual's relative risk for disease.
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