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COMPOSITIONS AND METHODS FOR TRANSCRIPTION-BASED CRISPR-CAS DNA EDITING
COMPOSITIONS AND METHODS FOR TRANSCRIPTION-BASED CRISPR-CAS DNA EDITING
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机译:基于转录的CRISPR-CAS DNA编辑的组合物和方法
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摘要
Provided are compositions and methods for cleaving a DNA sequence in a cell. The methods involve comprising introducing into a cell a recombinant vector containing a clustered regularly interspaced short palindromic repeats (CRISPR) system. The system includes a CRISPR RNA (crRNA) targeted to a DNA sequence in the cell that is operatively linked to a promoter; and CRISPR-associated enzymes (Cas) 10, Cas6, and at least one Csm protein. The Cas 10 cleaves the DNA sequence only during transcription of the DNA sequence that is operatively linked to the promoter. Also provided are recombinant vectors for modifying cells, cells that contain the recombinant vectors and modifications introduced by them, and kits that include the modified vectors.
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机译:提供了用于在细胞中切割DNA序列的组合物和方法。该方法包括将含有簇状规则间隔的短回文重复序列(CRISPR)系统的重组载体引入细胞。该系统包括针对细胞中DNA序列的CRISPR RNA(crRNA),该DNA序列可操作地连接至启动子。和CRISPR相关酶(Cas)10,Cas6和至少一种Csm蛋白。 Cas 10仅在与启动子可操作连接的DNA序列的转录期间才切割DNA序列。还提供了用于修饰细胞的重组载体,包含重组载体和由其导入的修饰的细胞以及包括修饰载体的试剂盒。
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