REDUCED REPRESENTATION BISULFITE SEQUENCING USING URACIL N-GLYCOSYLASE (UNG) AND ENDONUCLEASE IV

摘要

In a first aspect, embodiments disclosed herein provide methods for preparing a sample for sequencing, comprising: treating nucleic acid molecules in the sample to convert at least a portion of unmethylated cytosine residues into uracil residues; and cleaving the nucleic acid molecules at at least a portion of the uracil residues to obtain nucleic acid fragments. Further provided are populations of nucleic acid fragments resulting from a sample treated with the methods disclosed herein.