METHOD FOR PRE-TREATING SAMPLE SOLUTION FOR USE IN DETECTION AND QUANTIFICATION OF POLYETHYLENE GLYCOL-MODIFIED INTERFERON-β, METHOD FOR DETECTING POLYETHYLENE GLYCOL-MODIFIED INTERFERON-β, AND METHOD FOR QUANTIFYING POLYETHYLENE GLYCOL-MODIFIED INTERFERON-β

摘要

The purpose of the present invention is to provide a method for pre-treating a sample solution for use in the detection and quantification of polyethylene glycol-modified interferon-β in the sample solution, wherein polyethylene glycol-modified interferon-β and polyethylene glycol-unmodified interferon-β co-exist in the sample solution. The present invention provides a method for pre-treating a sample solution for use in the detection and quantification of polyethylene glycol-modified interferon-β, said method comprising: an acid treatment step of adding an acid to the sample solution to adjust the pH value of the sample solution to a value ranging from -0.10 to 0.50; and a coagulated protein removal step of removing a protein coagulated in the acid treatment step from the sample solution.