RECOMBINANT CORYNEBACTERIUM GLUTAMICUM IN WHICH CELLULASE COMPLEX IS IMMOBILIZED ON SURFACE OF CELLS, AND SACCHARIFICATION METHOD USING SAME

摘要

The present invention relates to: a recombinant Corynebacterium sp. microorganism in which a cellulase complex is immobilized on the surface of cells; and a method for decomposing biomass by using the same and, more specifically, to: a gene structure comprising (i) a gene encoding chimeric endo-β-1,4-glucanase E (chimera CelE) in which the active site of endo-β-1,4-glucanase E (CelE) and the dockerin site of endo-β-1,4-glucanase B (EngB) are linked, (ii) a Corynebacterium-derived membrane scaffolding protein (Msc) gene, and (iii) cellulosomal basic scaffolding subunit mini cellulose binding protein A (miniCbpA) gene; a recombinant Corynebacterium sp. microorganism to which a chimeric β-glucosidase gene, in which the gene structure, β-glucosidase gene and the dockerin site of EngB are linked, is introduced; and a method for decomposing biomass by using the microorganism. The recombinant Corynebacterium sp. microorganism in which a cellulase complex is immobilized on the surface, according to the present invention, has increased cellulase thermal stability and has increased biomass decomposition efficiency compared with that of an un-immobilized cellulase complex, and thus is useful for the production of a fermentation product.