METHOD FOR VISUALISING BIOMOLECULES, SUCH AS PROTEINS OR NUCLEIC ACIDS, WITH THE UNAIDED EYE, WITHOUT NEEDING TO USE POTENTIALLLY TOXIC COMPOUNDS, EXPOSURE TO ULTRAVIOLET (UV) LIGHT OR FLUORESCENCE

摘要

The present invention relates to a method for visualising biomolecules, which comprises the following steps: a) providing a sample of biomolecules immobilised in a matrix and performing electrophoresis; b) incubating the matrix from step a) in a solution that contains a molecule derived from cyanine, for a period of between 5 to 60 minutes, at room temperature, in a container that prevents exposure to light and agitating the container at less than 75 rpm; c) transferring the matrix from step b) to a container with a solution that comprises at least a tetrazolium salt, and incubating for a period of between 15 and 120 minutes, at room temperature and exposed to light; d) removing the matrix with immobilised biomolecules that was obtained from the previous step and washing with distilled water; and e) visualising the biomolecules immobilised in the matrix, directly with the unaided eye.