Use of UV Methods for Measurement of Protein and Nucleic Acid Concentrations

摘要

Measurement of absorbance at 260 nm is a well-established method for estimation of concentration of nucleic acids and oligomiclcolides in solution. Measurement at more than one wavelength, e.g., 260 and 280 nm, from which a ratio can be determined, is a check of the validity of the A_(260) reading. It is also a means of estimating whether the nucleic acid is contaminated with protein or other materials.