INTEGRATION OF BETA-ACTIN GENE FOR SAMPLE QUALITY CHECK IN HSV-1 AND HSV-2 DIAGNOSTIC KIT

摘要

Primers and probes for the in-vitro detection of Human HSV- 1 and HSV-2 virus in a sample comprising nucleotide sequences selected from the group comprising of or any combinations thereof: a nucleic acid molecule that encodes a nucleotide sequence of SEQ ID NO: l and SEQ ID No. 4 or a part or a nucleotide having minimum 90% sequence identity with said SEQ ID NO: l, wherein the nucleotide sequence of SEQ ID NO: l or SEQ ID No. 4 represents forward primer to amplify human HSV- 1 virus; a nucleic acid molecule that encodes a nucleotide sequence of SEQ ID NO: 2 and SEQ ID No. 5 or a part or a nucleotide having minimum 90% sequence identity with said SEQ ID NO:2 and SEQ ID No. 5, wherein the nucleotide sequence of SEQ ID NO: l represents reverse primer to amplify human HSV- 1; and a nucleic acid molecule that encodes a nucleotide sequence of SEQ ID NO:3 and SEQ ID NO. 6 or a part or a nucleotide having minimum 90% sequence identity with said SEQ ID NO:3 and SEQ ID No. 6, wherein the nucleotide sequence of SEQ ID NO:3 and SEQ ID No. 6 represents probes to detect human HSV- 1 and HSV-2. The nucleotide sequences enables high detection of all genotypes of Human HSV- 1 and HSV-2 wherein said nucleotide sequences enables detection of virus present in low copy number upto 102 IU/ml.