Provided is an equilibrium potential estimation method for a redox substance having a first stage for applying a voltage to an electrode in contact with a sample including the redox substance and sweeping the voltage, a second stage for measuring the current flowing through the electrode, a third stage for determining whether to sweep the voltage in a direction opposite from the sweeping direction of the previous sweeping stage or stop voltage sweeping when an accumulated current value falls within a standard range, a fourth stage for estimating the voltage value to be the equilibrium potential of the redox substance when a determination is made to stop voltage sweeping, and a fifth stage for sweeping the voltage in the direction opposite from the sweeping direction of the previous sweeping stage when a decision is made to sweep the voltage.
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