PRIMER FOR DETECTING COLORECTAL CANCER DRUG-RELATED GENE MUTATION AND DETECTION METHOD

摘要

Disclosed are a primer for detecting colorectal cancer drug-related gene mutation, a detection method and an application. The primer of the present application comprises 25 specific primer sets and 1 general primer set. The specific primer sets comprise specific primers respectively for detecting 27 mutation sites of 15 colorectal cancer drug-related genes, and the general primer set comprises outer primers commonly used for amplified products of the 25 specific primer sets. The 25 specific primer sets are sequentially shown as Seq ID NO. 1 to Seq ID NO. 50, wherein Seq ID NO. 1 and Seq ID NO. 2 form one set, Seq ID NO. 3 and Seq ID NO. 4 form one set, and so on; and the sequences of the general primer set are shown as Seq ID NO. 51 and Seq ID NO. 52.