EXOSOME ASSAY METHOD AND EXOSOME EXTRACTION METHOD

摘要

A specimen containing exosomes is mixed with a metal ion solution such as a gold ion solution to obtain a mixed solution. The mixed solution is examined for the amount of fine metal particles which have been aggregated therein, by means of absorbance, etc. The concentration of exosomes is evaluated from the amount of the aggregated fine metal particles. Meanwhile, gold ions are used as metal ions, and fine gold particles which have been aggregated in the mixed solution are sedimented by centrifuging. The supernatant is removed therefrom, and sodium thiosulfate is thereafter added dropwise to the residue. The mixture is centrifuged again to sediment the fine gold particles. The supernatant is recovered therefrom to extract the exosomes.