SOLANUM DUBIUM SEEDS AND HONEY (EXTRACTED FROM BEE HIVES IN ACACIA TREE POPULATED AREA) COMBINATION FOR TREATMENT OF ASTHMA AND BRONCHIAL ASTHMA

摘要

As a herbal remedy to asthma the invention is composed of weighed proportional mixture of Sunnut honey and powder of grounded seeds of Solatium, dubium. Phytochcmical analysis was performed on the crude extracts of the seeds, honey and their combination. Separation and isolation of active compounds from the methanolic extract of combination was carried out using bioassay-guided fractionation. Fractionation and isolation of the active ingredients of the methanolic extract were performed by Column Chromatography (monitored by TLC and PTLC). Nuclear magnetic resonance (NMR), mass spectrometry (MS), ultraviolet (UV) and infrared (IR) spectrometry were used for structure elucidation of the isolated compounds. The in-vivo experiments implemented are to assess the toxicological study of the crude and methanolic extracts of the combination, honey and the seeds on (96 )Wister Albino rats using the acute toxicity (Ll½ percentage) done on 7- days and sub-chronic for 28 days. The results revealed that there is no acute or sub-chronic toxicity displayed by the different concentrations of the extracts, The method of Carrageenan induced paw oedem in (162) Wister Rats was done to evaluate the anti-inflammatory effects of aqueous crude extract of S. dubuim seeds, honey and combination. The results showed that all ingredients were found to have a significant anti inflammatory activity on paw edema volume. Also the result of methanolic extracts showed a significant inhibition of inflammation. The results of the anti-inflammatory activity of the fractions of methanolic extract, five fractions showed a significant inhibition of inflammation over the other alcoholic fraction extracts. Also all fractions decreased rat paw edema. The bronchodilator activities of aqueous crude extract of the three ingredients was done using in vitro studies on isolated tracheal preparations of Guinea pigs and Guinea pigs pattern of allergic asthma setup experiment. The results of bronchodilator activities, the animal of control group, prior administration of the combination, lmg/ml partially blocked the contracting effect of histamine 100 nano g/ml on isolated tracheal spirally cut strip. The S. dubuim seeds extract alone produced further transient stimulant effect followed by persistent relaxant effect on histamine contracting effect on isolated guinea pigs tracheal strip. In animals of sensitized group, the combination and seeds extract lmg/ml relaxed effectively the stimulant effect of the guinea pigs tracheal strip. The relaxant effect of the combination was completely blocked by propranolal. The sensitized and treatment subgroups, both honey and combination antagonized the contracting effect of histamine 100 nano g/ml on guinea pigs tracheal strip, As a clinical trial for the combined ingredients, twenty five patients medically diagnosed as asthmatic were randomly selected and enrolled in the study; none of them is currently using anti-asthmatic drugs. Signs of asthma disappeared in the treated patients.