首页> 外国专利> METHOD FOR MASS PRODUCTION OF TULIP BULBLET THROUGH LIQUID CULTURE OF TULIP EMBRYOGENIC CALLUS IN BIOREACTOR AND REGENERATION ON SOLID MEDIA

METHOD FOR MASS PRODUCTION OF TULIP BULBLET THROUGH LIQUID CULTURE OF TULIP EMBRYOGENIC CALLUS IN BIOREACTOR AND REGENERATION ON SOLID MEDIA

机译:在生物反应器中通过郁金香胚性愈伤组织的液体培养大量生产郁金香郁金香的方法及在固体培养基上的再生

摘要

Provided is a mass production method of tulip bulblets using embryogenic calluses derived from the tulip bulb tissue. In regards to a method for massively increasing tulip bulblets by using somatic embryos induced from embryogenic calluses derived from the tulip bulb tissue, the present invention relates to a method for massively increasing tulip bulblets; to the tulip bulblets produced by the method; and to a liquid culture medium composition to massively increasing embryogenic calluses derived from the tulip bulb tissue. The method for massively increasing tulip bulblets comprises the steps of (1) massively increasing embryogenic calluses derived from tulip bulb tissue in a liquid culture medium; and (2) culturing the massively increased embryogenic calluses in a solid culture medium to form bulblets by somatic embryogenesis. The liquid culture medium composition contains, as a useful ingredient, a Murashige & Skoog (MS) culture medium including 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyl adenine (BA); or an MS culture medium including picloram and BA.
机译:提供了一种使用源自郁金香鳞茎组织的胚性愈伤组织的郁金香鳞茎的大量生产方法。关于通过使用由源自郁金香鳞茎组织的胚性愈伤组织诱导的体细胞胚来大量增加郁金香鳞茎的方法,本发明涉及一种大规模增加郁金香鳞茎的方法。用该方法生产的郁金香鳞茎;以及液体培养基组合物,以大量增加源自郁金香鳞茎组织的胚性愈伤组织。大量增加郁金香鳞茎的方法包括以下步骤:(1)在液体培养基中大量增加源自郁金香鳞茎组织的胚性愈伤组织; (2)在固体培养基中培养大量增加的胚性愈伤组织,以通过体细胞胚发生形成球根。液体培养基组合物含有Murashige&Skoog(MS)培养基作为有用成分,该培养基包括2,4-二氯苯氧基乙酸(2,4-D)和苄基腺嘌呤(BA);和或包含Picloram和BA的MS培养基。

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