Method for mass production of tulip bulblet through liquid culture of tulip embryogenic callus in bioreactor and regeneration on solid media

摘要

The present invention relates to a method for mass production of a tulip bulb using a somatic embryo derived from a embryogenic callus derived from a tulip bulb tissue, comprising the steps of: (1) massively propagating embryogenic callus derived from a tulip bulb tissue in a liquid medium; And (2) culturing the mass-propagated embryogenic callus in a solid medium to form a somite embryo. The method for mass production of tulip buds, the tulip produced by the method MS (Murashige & Skoog) medium containing magnetic domain and 2,4-D (2,4-dichlorophenoxyacetic acid) and BA (benzyl adenine); The present invention relates to a liquid medium composition for mass-proliferating embryogenic calli derived from tulip bulb tissues containing, as an active ingredient, MS medium containing picloram and BA.