METHOD FOR QUANTITATIVE ANALYSIS OF FLT3 GENE MUTATION AND KIT

摘要

The present invention relates to a method for quantitatively analyzing a mutation on fms-related tyrosine kinase 3 (FLT3) gene, and to a kit for the same. According to the present invention, the FLT3-internal tandem duplication (ITD) quantitative analytic method using a fragment analysis is more economical than a real-time quantitative polymerase chain reaction (PCR) method, and the method ensures effective measurement of a quantitative value of the mutation which shows various aspacts like the FLT3-ITD in a relatively easy way. In addition, the method ensures excellent specificity and sensitivity than an outcome derived from an existing PCR, and thus the method can be useful for analyzing the quantitative value of FLT3-ITD in a patient with acute myelocyte leukemia. Further, the method is highly correlated with the prognosis, thereby being useful clinically as well.