METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN BRAF, NRAS AND KIT GENES USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP)

摘要

FIELD: biology; medicine.;SUBSTANCE: invention relates to the field of genetics, molecular biology and medicine. Method for detecting somatic mutations in the BRAF, NRAS and KIT genes is proposed. Carry out the amplification of gene fragments BRAF, NRAS and KIT using LNA-blocking multiplex "nest" PCR. Biochip is used to identify somatic mutations in the BRAF, NRAS and KIT genes and hybridize the labeled PCR product on the biochip. Record the results of hybridization.;EFFECT: invention provides a method for determining somatic mutations in the BRAF, NRAS and KIT genes that affect the sensitivity of melanoma to targeted therapy.;3 cl, 2 dwg, 4 ex