首页> 外国专利> METHOD FOR PRODUCING EPIDERMAL GROWTH FACTOR PROTEIN BY USING TRANSFORMATION SECRETION VECTOR CONTAINING LACCASE PROMOTER OF PLEUROTUS ERYNGII

METHOD FOR PRODUCING EPIDERMAL GROWTH FACTOR PROTEIN BY USING TRANSFORMATION SECRETION VECTOR CONTAINING LACCASE PROMOTER OF PLEUROTUS ERYNGII

机译:包含杏鲍菇杆状酶启动子的转化分泌载体生产表皮生长因子蛋白的方法

摘要

The present invention relates to a Pleurotus eryngii transformation secretion vector and a method for producing an epidermal growth factor protein by using the same. More specifically, the present invention provides a secretion vector including an expression construct suitable for mushroom transformation by separating a laccase promoter and a signal sequence gene from Pleurotus eryngii, and a method for successfully producing various target proteins such as an epidermal growth factor protein from a transformed mushroom by using the same. A recombinant expression vector according to the present invention may be contributively utilized for a system for mass-producing various industrially useful proteins in a mushroom, particularly, in Pleurotus eryngii.;COPYRIGHT KIPO 2016
机译:本发明涉及杏鲍菇转化分泌载体和使用其生产表皮生长因子蛋白的方法。更具体地,本发明提供了一种分泌载体,其包括适于通过从杏鲍菇分离漆酶启动子和信号序列基因而适于蘑菇转化的表达构建体,以及一种成功地从大肠杆菌中产生各种靶蛋白如表皮生长因子蛋白的方法。通过使用相同的蘑菇。根据本发明的重组表达载体可以用于在蘑菇中,特别是在杏鲍菇中大量生产各种工业上有用的蛋白质的系统。COPYRIGHTKIPO 2016

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