首页> 外国专利> SYNTHESIS OF SLOWLY DIGESTIBLE STARCH BY MALTOSE-BINDING-PROTEIN FUSED 4,6-ALPHA-GLUCANOTRANSFERASE AND THE USES THEREOF

SYNTHESIS OF SLOWLY DIGESTIBLE STARCH BY MALTOSE-BINDING-PROTEIN FUSED 4,6-ALPHA-GLUCANOTRANSFERASE AND THE USES THEREOF

机译:麦芽糖结合蛋白融合的4,6-α-葡聚糖转移酶的慢消化淀粉合成及其用途

摘要

The present invention provides a method for producing slowly digestible starch, and a food produced by using slowly digestible starch produced through the method. The method comprises the steps of: producing a recombinant vector including a maltose-binding-protein coding gene and a gene for coding a 4,6GT, 4,6--glucanotransferase derived from Lactobacillus reuteri; transforming the produced recombinant vector to a colon bacillus; separating and refining maltose-binding-protein fused 4,6GT, 4,6--glucanotransferase from the transformed colon bacillus; and reacting the refined maltose-binding-protein fused 4,6--glucanotransferase with starch.
机译:本发明提供了一种生产缓慢消化的淀粉的方法,以及一种使用通过该方法生产的缓慢消化的淀粉生产的食品。该方法包括以下步骤:产生重组载体,该重组载体包括麦芽糖结合蛋白编码基因和用于编码来自罗伊氏乳杆菌的4,6GT,4,6-葡聚糖转移酶的基因。将产生的重组载体转化为结肠杆菌;从转化的结肠杆菌中分离和提纯麦芽糖结合蛋白融合的4,6GT,4,6-葡聚糖转移酶;使精制的麦芽糖结合蛋白融合的4,6-葡聚糖转移酶与淀粉反应。

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