Quick and effective confirmation of a genotype is important to maintain a mouse model indicating homozygous sterility. A leptin receptor mutation (C57BL/KsJ-db/db) mouse indicating homozygous sterility is generally used as a non-insulin dependent diabetic animal model. Inventors use a tetra-primer ARMS-PCR method for the quick and effective genotype confirmation of a C57BL/KsJ-db/db mouse. 407 bp and 199 bp PCR products are amplified from a normal (+/+) mouse; while 407 bp and 268 bp PCR products are amplified from a homozygous (db/db) mouse; and 497 bp, 268 bp, and 199 bp PCR products are amplified from a heterozygote (db/+) mouse. Furthermore, a tetra-primer ARMS-PCR analysis method is effective for a different mouse having a C57BL/6J gene background. The tetra-primer ARMS-PCR analysis method is effective for system-maintaining C57BL/KsJ-db/db; for mass-producing homozygous (db/db) mice to be used for experiments; and for quickly mass-producing a double knockout mouse containing leptin receptor mutation.;COPYRIGHT KIPO 2016
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