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CULTURE MEDIUM COMPOSITION FOR CULTURING MOUSE GLIAL CELLS, AND METHOD FOR INDUCING DIFFERENTIATION OF MOUSE GLIAL CELLS
CULTURE MEDIUM COMPOSITION FOR CULTURING MOUSE GLIAL CELLS, AND METHOD FOR INDUCING DIFFERENTIATION OF MOUSE GLIAL CELLS
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机译:培养小鼠神经胶质细胞的培养培养基组合物和诱导小鼠神经胶质细胞分化的方法
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摘要
The present invention relates to a culture medium composition for culturing mouse glial cells, and to a method for inducing the differentiation of mouse glial cells. According to the present invention, 20 to 80 ug/mL of transferrin, 1 to 10 ng/mL of progesterone, 10 to 18 ug/mL of putrescine, 1 to 8 ng/mL of sodium selenite, 1 to 5 mM of glutamine, 1 to 10 ug/mL of insulin, 0.1 to 1.5 ug/mL of thyroxin, and 1 to 10 ug/mL of glucose are included in a Dulbecco′s modified Eagle′s medium (DMEM). Accordingly, the differentiation of glial cells, for example, oligodendrocytes can be induced from mouse cortex precursor cells.;COPYRIGHT KIPO 2016
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机译:本发明涉及用于培养小鼠神经胶质细胞的培养基组合物,以及诱导小鼠神经胶质细胞分化的方法。根据本发明,20至80 ug / mL的转铁蛋白,1至10 ng / mL的孕酮,10至18 ug / mL的腐胺,1至8 ng / mL的亚硒酸钠,1至5 mM的谷氨酰胺, Dulbecco®改良的Eagle®培养基(DMEM)中包含1至10 ug / mL的胰岛素,0.1至1.5 ug / mL的甲状腺素和1至10 ug / mL的葡萄糖。因此,可以从小鼠皮质前体细胞诱导神经胶质细胞的分化,例如少突胶质细胞。; COPYRIGHT KIPO 2016
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