- Detection of Target Nucleic Acid Sequences Using Dual-Labeled Immobilized Probes on Solid Phase

摘要

the present invention is dual-labeled immobilized probe, and to a novel method for detecting a target nucleic acid sequence in solid phase using a resistance to the active 5 ' 3' exonuclease of his DNA polymerase It relates. The present invention, because the cover remaining on the solid phase substrate by resistance to nucleases due to the cover of the base component of the internal nucleotide, the labeling on the solid phase substrate need not be considered a suitable location to the residual. The present invention can freely determine the position of the inner cover on the probe, to maximize the efficiency of quenching of the probe onto the double cover system sikimeuroseo position the cover in the appropriate location (site), minimizing the background signal. ;