Suicide vector for producing murtant strain of multidrug-resistant strain

摘要

The present invention relates to a suicide vector which is not replicated in a multidrug-resistant strain but can be used for producing a mutant strain of a multidrug-resistant strain, a kit for producing a mutant strain of a multidrug-resistant strain containing the suicide vector, A method for producing a mutant strain in which a gene is deleted, and a mutant strain produced by the above method and having deletion of a target gene of a multidrug-resistant strain. Using the kit for producing a mutant strain of a suicide vector or a multidrug resistant strain for producing a mutant strain of a multidrug-resistant strain provided by the present invention, even when only a limited antibiotic resistance gene that can be used for studying various multidrug resistant strains can be used, Since a mutant strain in which one or more target genes or target polynucleotides are deleted from the genomic DNA can be produced, it can be widely used in various fields such as genome and physiological activity of various multidrug-resistant strains.