A novel scanning electron microscopic tissue processing method for diagnosis of malignant mesothelioma using paraffin embedded pathologic specimen

摘要

The present invention relates to a specimen making method for scanning electron microscopic observation of pathological specimens, including the novel mesothelioma diagnostic paraffin tissue, through more specifically microvilli observed located between tumor cells It relates to a method for manufacturing samples for scanning electron microscopic observation of pathological specimens, including diagnosis of a tumor tissue paraffin to accurately diagnose mesothelioma. According to the present invention, by utilizing a paraffin remaining tissue of the tumor tissue to easily create for a biopsy sample in a short time. Limit of the resolving power when a paraffin produced by a conventional method was observed with an optical microscope (20-fold to 1,000-fold) in tumor cells observing the region to be faintly observed in the gap with excellent scanning electron microscope resolution (1000-fold to 10,000 since times) if thin and can be clearly observed long microvilli, possibly other tumor cells and differentiation, particularly tissue production for scanning electron microscopy utilizing paraffin remaining tissue when ambiguous immunohistochemical results confirmed by the characteristic shape of the microvilli through the process it will be utilized to make useful diagnostic mesothelioma. ;