NUCLEIC ACID ABUNDANCE RATIO MEASUREMENT DEVICE METHOD AND PROGRAM STORAGE MEDIUM DETERMINATION METHOD AND NECLEIC ACID ABUNDANCE RATIO MEASUREMENT KIT

摘要

[PROBLEMS] To provide an apparatus for measuring the abundance ratio of nucleic acid, a method for measuring the abundance ratio of nucleic acid, a program for measuring the abundance ratio of nucleic acid, and a kit for measuring the abundance ratio of nucleic acid. [MEANS FOR SOLVING PROBLEMS] Each of melting temperatures (Tm _W and Tm) of different sizes based on a differential melting curve showing the relationship between the respective temperatures of a plurality of nucleic acid mixtures with different abundance ratios of two kinds of nucleic acids and differential values of fluorescence intensity relationship with _M) 2 of the temperature range (ΔT _W and ΔT _M) area ratio of the portion of the differential melting surrounded by a line indicative of the curve and the base value, within (S _M / S _W) with the two types of the nucleic acid abundance containing Is stored in the memory 26 as shown in FIG. (S ' _M / S' _W ) of a portion surrounded by a straight line indicating a differential melting curve and a base value within the two temperature ranges (ΔT ' _W and ΔT' _M ) of the differential melting curve of the nucleic acid mixture to be measured And calculating the abundance ratio of the nucleic acid based on the calculated area ratio and the calibration curve, thereby measuring the abundance ratio of the nucleic acid in the specimen.