Primer set for detection of Clostridium botulinum composition and kit comprising the same

摘要

The present invention is Clostridium botulinum (ClostridiumThe present invention relates to a primer set for a polymerase chain reaction of a light chain region coding gene of a toxin protein C of botulinum, a composition for detecting Botulinum toxin small C / D and C, and a detection kit, Clostridiumbotulinum) When performing the single-tube double polymerase chain reaction with oligonucleotide for poison small C / D and primers commonly coupled to the gene encoding the N-terminus of the toxin protein C and non-specific reaction inhibition using the nucleotides, botulinum The sensitivity can be improved so that the sample can be carried out without a microbial process by showing a lower detection limit, thereby enabling rapid diagnosis.