Detection, Isolation and analysis of rare cells in biological fluids

摘要

1. A process for the isolation or enrichment of rare cells from a biological fluid of a mammal, said method comprising: providing an antibody immobilized on a substrate, wherein said antibody binds to a surface antigen of rare cells, contacting said immobilized antibody with a sample of biological fluid, said biological fluid comprises a plurality of rare cell and other cells; implementation incubating said immobilized antibody with said sample biologist nical fluid under conditions suitable for binding of the antibody to the surface antigen of rare cells to form an antibody - a rare cell and the plurality of unbound cells; iotmyvku complex antibody - a rare cell to remove non-bound cells and provide complex antibody - kletka.2 rare. The method of claim. 1, characterized in that the said antibody is specific for a surface antigen fetal kletki.3. A method according to claim. 2, characterized in that said embryonic cell surface antigen is an antigen fetal nucleated eritrotsita.4. A method according to claim. 3, characterized in that said antibody is an antibody 4V9.5. The method of claim. 1, characterized in that said mammal is a cheloveka.6. The method of claim. 1, characterized in that said biological fluid is blood, plasma, amniotic fluid, urine or cell suspension of chorionic villus sampling (CVS) .7. A method for detecting rare cells in a biological fluid, said method comprising: to provide