CONSTRUCTIONS OF RECOMBINANT NON-PATHOGENIC MIRECA DISEASE VIRUS CODING ANTIGENS OF INFECTIOUS LARINGOTHARAHITIS VIRUS AND NEWcastle DISEASE VIRUS

摘要

1. Recombinant non-pathogenic Marek's disease virus (rMDV) containing the first nucleic acid inserted into the first, unnecessary portion in the rMDV genome, and the second nucleic acid inserted into the second, unnecessary portion in the rMDV genome; where the first nucleic acid contains both the nucleotide sequence encoding the gD protein of the infectious laryngotracheitis virus (ILTV) and the nucleotide sequence encoding the gD protein of the infectious laryngotracheitis virus gI (ILTV); where the second nucleic acid contains um the nucleotide sequence encoding a fusion protein of the Newcastle disease virus (NDV F); where the first, non-necessary site, and the second, non-necessary site, are either the same, or only the first, non-necessary site, is US2.2. rMDV of claim 1, wherein the first, non-essential portion, and the second, non-essential portion, are US2.3. rMDV according to claim 1, wherein the first, non-essential portion, and the second, non-essential portion, are UL54.5.4. rMDV according to claim 1, wherein the first, non-essential portion is US2, and the second, non-essential portion is UL7 / 8.5. rMDV according to claim 1, wherein the nucleotide sequence encoding an ILTV gD protein is functionally controlled by the first promoter, the nucleotide sequence encoding an ILTV gI protein is functionally controlled by a second promoter, and the nucleotide sequence encoding an NDV F protein is functionally controlled by a third promoter. 6. rMDV�